Antibodies, A Laboratory Manual, Second Edition, Edited by Edward A. Greenfield



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Detecting -Galactosidase-Labeled Cells Using X-Gal

(Protocol summary only for purposes of this preview site)

-Galactosidase has been used extensively both as a label in enzyme immunoassays and for immunocytochemistry. One good substrate is 5-bromo-4-chloro-3-indolyl--D-galactopyranoside (X-gal), which gives an intense blue product. The product is stable and insoluble in alcohol as well as H2O.

Antibodies: A Laboratory Manual, Second edition
Antibodies: A Laboratory Manual, Second edition
Antibodies: A Laboratory Manual, Second edition

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